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Main sol. 548

Internal Solution-ID: S1210

Compound Amount Unit Conc. [g/L] Conc. [mM]
Beef extract 1 g 1 -
Glucose 10 g 10 55.506
N-Z amine
(enzymatic digest of casein)
2 g 2 -
Yeast extract 1 g 1 -
Agar 20 g 15 44.599
Distilled water 1000 ml - -
1 Adjust pH to 7.3 with NaOH.
2 Modified Bennett's agar can also be used. In it, glucose is replaced by glycerol (10.0 g) and N-Z amine by Bacto casein (2.0 g, Difco).
548 BENNETT'S AGAR