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JCM 854 . COLON EXTRACT MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J854 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 167.0 ml - -
Solution B 167.0 ml - -
Solution C 40.0 ml - -
Solution D 200.0 ml - -
Yeast extract 5.0 g 3.157 -
Trypticase peptone 5.0 g 3.157 -
NaHCO3 5.0 g 3.157 37.575
Resazurin 1.0 mg 6.3e-4 0.003
L-Cysteine HCl x H2O 1.0 g 0.631 -
Vitamin K1
(0.5%) 		0.2 ml - -
Hemin solution 10.0 ml - -
Distilled water 1000.0 ml - -
1 Adjust pH to 7.0.

Solution A #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 5.40 g 5.4 92.403
KH2PO4 2.70 g 2.7 19.84
CaCl2 x 2 H2O 0.16 g 0.16 1.088
MgCl2 x 6 H2O 0.12 g 0.12 0.59
MnSO4 x n H2O 0.07 g 0.07 -
CoCl2 x 6 H2O 0.06 g 0.06 0.462
(NH4)2SO4 5.40 g 5.4 40.866
FeSO4 x 7 H2O 0.05 g 0.05 0.18
Distilled water 1000.00 ml - -

Solution B #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.7 g 2.7 15.502
Distilled water 1000.0 ml - -

Solution C #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 50 g 50 277.531
Maltose 50 g 50 146.071
Cellobiose 50 g 50 146.071
Xylose 50 g 50 333.045
Distilled water 1000 ml - -

Solution D #

Equipment needed: Centrifuge Filter
1 Dilute (1:1, w/v) the content of terminal pig colon with the following buffer: 167 ml Solution A (see above) and 167 ml Solution B (see above). Make up to 1.0 L with distilled water. Stomach the suspension and filter through a cheese cloth. Incubate at 37C for 48 h and adjust pH to 7.0. Centrifuge at 20,000 x g for 20 min. Retrieve the supernatant and store at -20C until use.

Hemin solution #

Solution occurs in 5 other media (first defined in Medium J469).
1 Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml.

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Centrifuge, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.157 37.575
Trypticase peptone 3.157 -
Yeast extract 3.157 -
Xylose 1.263 8.41
Glucose 1.263 7.008
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