854 .
COLON EXTRACT MEDIUM
What is this page?
Equipment needed:
Centrifuge
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 167.0 | ml | ||
|
|
Solution B | 167.0 | ml | ||
|
|
Solution C | 40.0 | ml | ||
|
|
Solution D | 200.0 | ml | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
Trypticase peptone | 5.0 | g | ||
|
|
NaHCO3 | 5.0 | g | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
L-Cysteine HCl x H2O | 1.0 | g | ||
|
|
Vitamin K1
(0.5%) |
0.2 | ml | ||
|
|
Hemin solution | 10.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.0. | |||||
Solution A #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 5.40 | g |
|
|
KH2PO4 | 2.70 | g |
|
|
CaCl2 x 2 H2O | 0.16 | g |
|
|
MgCl2 x 6 H2O | 0.12 | g |
|
|
MnSO4 x n H2O | 0.07 | g |
|
|
CoCl2 x 6 H2O | 0.06 | g |
|
|
(NH4)2SO4 | 5.40 | g |
|
|
FeSO4 x 7 H2O | 0.05 | g |
|
|
Distilled water | 1000.00 | ml |
Solution C #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Glucose | 50 | g |
|
|
Maltose | 50 | g |
|
|
Cellobiose | 50 | g |
|
|
Xylose | 50 | g |
|
|
Distilled water | 1000 | ml |
Solution D #
| 1 Dilute (1:1, w/v) the content of terminal pig colon with the following buffer: 167 ml Solution A (see above) and 167 ml Solution B (see above). Make up to 1.0 L with distilled water. Stomach the suspension and filter through a cheese cloth. Incubate at 37C for 48 h and adjust pH to 7.0. Centrifuge at 20,000 x g for 20 min. Retrieve the supernatant and store at -20C until use. |