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JCM 675 . RUMINOCOCCUS ALBUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Blautia wexlerae DSM 19850

This strain uses the standard medium without modifications.

Main sol. J675 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tryptone
(BD-Difco) 		5 g 5 70.343
Yeast extract
(BD-Difco) 		2 g 2 -
Glucose 3 g 3 16.652
Cellobiose 2 g 2 5.843
S I solution 6.0 g/L 40 ml - -
Mineral solution 2 40 ml - -
Resazurin 1 mg 1.0e-3 0.004
Distilled water 920 ml - -
1 Boil and cool under CO2. Under a gentle stream of CO2, add 4.0 g Na2CO3, 1.0 ml fatty acid mixture (see below) and 500 mg L-cysteine x HCl x H2O.
2 Adjust pH to 7.0, distribute into appropriate vessels under N2 and autoclave.

S I solution 6.0 g/L #

100
Solution occurs in 1 other media (first defined in Medium J674).
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.6 g 6 34.448
Distilled water 100.0 ml - -

Mineral solution 2 #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.60 g 6 44.09
(NH4)2SO4 2.00 g 20 151.355
NaCl 1.20 g 12 205.339
MgSO4 x 7 H2O 0.25 g 2.5 10.143
CaCl2 x 2 H2O 0.16 g 1.6 10.883
Distilled water 100.00 ml - -

Fatty acid mixture #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Isobutyric acid 10 ml - -
Isovaleric acid 10 ml - -
2-Methylbutyric acid 10 ml - -
Distilled water 70 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 6
Blautia wexlerae DSM 19850

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 5 70.343
Glucose 3 16.652
Cellobiose 2 5.843
Yeast extract 2 -
(NH4)2SO4 0.8 6.054
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Cultivation metadata from 6 strains BacDive

pH 6 - 8 1 strain
phyla 1 6 strains
oxygen anaerobe 5 strains
oxygen obligate anaerobe 1 strain
temperature mesophilic 6 strains