# J675: RUMINOCOCCUS ALBUS MEDIUM


## Main sol. J675

5	g	Tryptone (BD-Difco)	
2	g	Yeast extract (BD-Difco)	
3	g	Glucose	
2	g	Cellobiose	
40	ml	**S I solution 6.0 g/L**	
40	ml	**Mineral solution 2**	
1	mg	Resazurin	
920	ml	Distilled water	

1. Boil and cool under CO2. Under a gentle stream of CO2, add 4.0 g Na2CO3, 1.0 ml fatty acid mixture (see below) and 500 mg L-cysteine x HCl x H2O.
2. Adjust pH to 7.0, distribute into appropriate vessels under N2 and autoclave.


## S I solution 6.0 g/L

0.6	g	K2HPO4	
100	ml	Distilled water	


## Mineral solution 2

0.6	g	KH2PO4	
2	g	(NH4)2SO4	
1.2	g	NaCl	
0.25	g	MgSO4 x 7 H2O	
0.16	g	CaCl2 x 2 H2O	
100	ml	Distilled water	


## Fatty acid mixture

10	ml	Isobutyric acid	
10	ml	Isovaleric acid	
10	ml	2-Methylbutyric acid	
70	ml	Distilled water