J674
674 .
PYG MEDIUM (F)
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Ruminiclostridium sufflavum DSM 19573
This strain uses the standard medium without modifications.
Main sol. J674 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| S I solution 6.0 g/L | 75.0 | ml | - | - | |
| S II solution | 75.0 | ml | - | - | |
| Trypticase peptone | 10.0 | g | 9.96 | - | |
| Yeast extract | 5.0 | g | 4.98 | - | |
| Glucose | 10.0 | g | 9.96 | 55.285 | |
|
Sodium resazurin
(w/v, 0.1%) |
1.0 | ml | - | - | |
|
Na2CO3
(w/v, 8%) |
2.5 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.3 | g | 0.299 | - | |
| Distilled water | 850.0 | ml | - | - | |
| 1 Adjust pH to 7.3. | |||||
Solution occurs in 1
other media (first defined in Medium J674).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| K2HPO4 | 0.6 | g | 6 | 34.448 |
| Distilled water | 100.0 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 0.60 | g | 6 | 44.09 |
| (NH4)2SO4 | 1.20 | g | 12 | 90.813 |
| NaCl | 1.20 | g | 12 | 205.339 |
| MgSO4 x 7 H2O | 0.12 | g | 1.2 | 4.869 |
| CaCl2 x 2 H2O | 0.12 | g | 1.2 | 8.162 |
| Distilled water | 100.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 15 |
Ruminiclostridium sufflavum DSM 19573
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 9.96 | 55.285 |
| Trypticase peptone | 9.96 | - |
| Yeast extract | 4.98 | - |
| NaCl | 0.896 | 15.339 |
| (NH4)2SO4 | 0.896 | 6.784 |
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