# J674: PYG MEDIUM (F)


## Main sol. J674

75	ml	**S I solution 6.0 g/L**	
75	ml	**S II solution**	
10	g	Trypticase peptone	
5	g	Yeast extract	
10	g	Glucose	
1	ml	Sodium resazurin (w/v, 0.1%)	
2.5	ml	Na2CO3 (w/v, 8%)	
0.3	g	L-Cysteine HCl x H2O	
850	ml	Distilled water	

1. Adjust pH to 7.3.


## S I solution 6.0 g/L

0.6	g	K2HPO4	
100	ml	Distilled water	


## S II solution

0.6	g	KH2PO4	
1.2	g	(NH4)2SO4	
1.2	g	NaCl	
0.12	g	MgSO4 x 7 H2O	
0.12	g	CaCl2 x 2 H2O	
100	ml	Distilled water