J292
292 .
LACTOBACILLUS PONTIS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J292 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Lactobacilli MRS broth
(BD-Difco) |
55 | g | 55 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 6.5. | |||||
| 2 After autoclaving, cool to 50C and add aseptically 18 ml Maltose-hemin solution. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Maltose | 15 | g | 100 | 292.141 | |
| Hemin solution | 50 | ml | - | - | |
| L-Cysteine HCl x H2O | 5 | g | 33.333 | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Filter-sterilize. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Hemin chloride | 0.1 | g | 1 | - | |
| Triethanolamine | 4.0 | ml | - | - | |
| Distilled water | 96.0 | ml | - | - | |
| 1 After filter-sterilizing, store at 4C. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Lactobacilli MRS broth | 55 | - |
| Distilled water | - | - |
