292 .
LACTOBACILLUS PONTIS MEDIUM
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Equipment needed:
Filter
Final pH:
6.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Lactobacilli MRS broth
(BD-Difco) |
55 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 6.5. | |||||
| 2 After autoclaving, cool to 50C and add aseptically 18 ml Maltose-hemin solution. | |||||
Maltose-hemin solution #
150
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Maltose | 15 | g | ||
|
|
Hemin solution | 50 | ml | ||
|
|
L-Cysteine HCl x H2O | 5 | g | ||
|
|
Distilled water | 100 | ml | ||
| 1 Filter-sterilize. | |||||
Hemin solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Hemin chloride | 0.1 | g | ||
|
|
Triethanolamine | 4.0 | ml | ||
|
|
Distilled water | 96.0 | ml | ||
| 1 After filter-sterilizing, store at 4C. | |||||