# J292: LACTOBACILLUS PONTIS MEDIUM


## Main sol. J292

55	g	Lactobacilli MRS broth (BD-Difco)	
1000	ml	Distilled water	

1. Adjust pH to 6.5.
2. After autoclaving, cool to 50C and add aseptically 18 ml Maltose-hemin solution.


## Maltose-hemin solution

15	g	Maltose	
50	ml	**Hemin solution**	
5	g	L-Cysteine HCl x H2O	
100	ml	Distilled water	

1. Filter-sterilize.


## Hemin solution

0.1	g	Hemin chloride	
4	ml	Triethanolamine	
96	ml	Distilled water	

1. After filter-sterilizing, store at 4C.