J1130
1130 .
YCFA MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Oliverpabstia intestinalis DSM 106162
This strain uses the standard medium without modifications.
Main sol. J1130 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone
(BD-Difco) |
10.0 | g | 10.235 | 143.999 | |
|
Yeast extract
(BD-Difco) |
2.5 | g | 2.559 | - | |
| NaHCO3 | 4.0 | g | 4.094 | 48.736 | |
| Glucose | 2.0 | g | 2.047 | 11.363 | |
| Maltose | 2.0 | g | 2.047 | 5.98 | |
| Cellobiose | 2.0 | g | 2.047 | 5.98 | |
| L-Cysteine HCl x H2O | 1.0 | g | 1.024 | - | |
| Mineral solution I | 150.0 | ml | - | - | |
| Mineral solution II | 150.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 0.001 | 0.004 | |
| VFA mix | 6.2 | ml | - | - | |
| Hemin solution | 10.0 | ml | - | - | |
| Vitamin solution I | 1.0 | ml | - | - | |
| Distilled water | 660.0 | ml | - | - | |
| 1 Adjust pH to 7.45. | |||||
| 2 After autoclaving, add filter-sterilized 1.0 ml Vitamin solution II (see below). | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| K2HPO4 | 3 | g | 3 | 17.224 |
| Distilled water | 1000 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 3.0 | g | 3 | 22.045 |
| (NH4)2SO4 | 6.0 | g | 6 | 45.406 |
| NaCl | 6.0 | g | 6 | 102.669 |
| MgSO4 x 7 H2O | 0.6 | g | 0.6 | 2.434 |
| CaCl2 x 2 H2O | 0.6 | g | 0.6 | 4.081 |
| Distilled water | 1000.0 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Acetic acid | 17 | ml | - | - |
| Propionic acid | 6 | ml | - | - |
| n-Valeric acid | 1 | ml | - | - |
| iso-Valeric acid | 1 | ml | - | - |
| iso-Butyric acid | 1 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Biotin | 5 | mg | 0.005 | 0.02 | |
| Vitamin B12 | 5 | mg | 0.005 | 0.004 | |
| p-Aminobenzoic acid | 15 | mg | 0.015 | 0.109 | |
| Folic acid | 25 | mg | 0.025 | 0.057 | |
| Pyridoxine hydrochloride | 75 | mg | 0.075 | - | |
| Distilled water | 500 | ml | - | - | |
| 1 Vitamin solution II: | |||||
| Thiamine HCl | 25 | mg | 0.025 | - | |
| Riboflavin | 25 | mg | 0.025 | 0.066 | |
| Distilled water | 500 | ml | - | - | |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.4 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 66 |
Oliverpabstia intestinalis DSM 106162
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Tryptone | 10.235 | 143.999 |
| NaHCO3 | 4.094 | 48.736 |
| Yeast extract | 2.559 | - |
| Glucose | 2.047 | 11.363 |
| Cellobiose | 2.047 | 5.98 |
| Show more | ||
Cultivation metadata from 66 strains
| pH | 6 - 8 | 4 strains |
| pH | 4 - 6 | 3 strains |
| phyla | 4 | 67 strains |
| oxygen | anaerobe | 26 strains |
| oxygen | obligate anaerobe | 4 strains |
| Show more | ||