# J1130: YCFA MEDIUM ## Main sol. J1130 10 g Tryptone (BD-Difco) 2.5 g Yeast extract (BD-Difco) 4 g NaHCO3 2 g Glucose 2 g Maltose 2 g Cellobiose 1 g L-Cysteine HCl x H2O 150 ml **Mineral solution I** 150 ml **Mineral solution II** 1 mg Resazurin 6.2 ml **VFA mix** 10 ml **Hemin solution** 1 ml **Vitamin solution I** 660 ml Distilled water 1. Adjust pH to 7.45. 2. After autoclaving, add filter-sterilized 1.0 ml Vitamin solution II (see below). ## Mineral solution I 3 g K2HPO4 1000 ml Distilled water ## Mineral solution II 3 g KH2PO4 6 g (NH4)2SO4 6 g NaCl 0.6 g MgSO4 x 7 H2O 0.6 g CaCl2 x 2 H2O 1000 ml Distilled water ## VFA mix 17 ml Acetic acid 6 ml Propionic acid 1 ml n-Valeric acid 1 ml iso-Valeric acid 1 ml iso-Butyric acid ## Hemin solution 0.28 g KOH 25 ml Ethanol 0.1 g Hemin 1. Adjust volume to 100 ml with distilled water. ## Vitamin solution I 5 mg Biotin 5 mg Vitamin B12 15 mg p-Aminobenzoic acid 25 mg Folic acid 75 mg Pyridoxine hydrochloride 500 ml Distilled water 1. Vitamin solution II: 25 mg Thiamine HCl 25 mg Riboflavin 500 ml Distilled water