Main sol. J1130
Internal Solution-ID: S5225
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone
(BD-Difco) |
10.0 | g | 10.235 | 143.999 | |
|
Yeast extract
(BD-Difco) |
2.5 | g | 2.559 | - | |
| NaHCO3 | 4.0 | g | 4.094 | 48.736 | |
| Glucose | 2.0 | g | 2.047 | 11.363 | |
| Maltose | 2.0 | g | 2.047 | 5.98 | |
| Cellobiose | 2.0 | g | 2.047 | 5.98 | |
| L-Cysteine HCl x H2O | 1.0 | g | 1.024 | - | |
| Mineral solution I | 150.0 | ml | - | - | |
| Mineral solution II | 150.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 0.001 | 0.004 | |
| VFA mix | 6.2 | ml | - | - | |
| Hemin solution | 10.0 | ml | - | - | |
| Vitamin solution I | 1.0 | ml | - | - | |
| Distilled water | 660.0 | ml | - | - | |
| 1 Adjust pH to 7.45. | |||||
| 2 After autoclaving, add filter-sterilized 1.0 ml Vitamin solution II (see below). | |||||
Media with this solution
| J1130 | YCFA MEDIUM |