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JCM 1088 . 0.3 x HYPHOMICROBIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1088 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.85 g 0.846 4.856
NaH2PO4 0.75 g 0.746 6.22
(NH4)2SO4 0.20 g 0.199 1.506
MgSO4 x 7 H2O 0.07 g 0.07 0.283
Visniac trace elements 1.00 ml - -
Trace vitamins 2.00 ml - -
Methanol 2.00 ml - -
Agar 15.00 g 14.925 44.377
Distilled water 1000.00 ml - -
1 After autoclaving, aseptically add filter-sterilized trace vitamins solution and methanol.

Trace vitamins #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 125.0 mg 0.25 0.184
Thiamine HCl 25.0 mg 0.05 -
Folic acid 12.5 mg 0.025 0.057
Ascorbic acid 40.0 mg 0.08 0.454
Riboflavin 25.0 mg 0.05 0.133
Niacin 25.0 mg 0.05 0.406
Pantothenic acid 25.0 mg 0.05 0.228
Distilled water 500.0 ml - -

Visniac trace elements #

500
Solution occurs in 1 other media (first defined in Medium J884).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.000 g 10 -
ZnSO4 x 7 H2O 2.200 g 4.4 15.301
CaCl2 x 2 H2O 0.733 g 1.466 9.972
MnCl2 x 4 H2O 0.506 g 1.012 -
FeSO4 x 7 H2O 0.499 g 0.998 3.59
(NH4)6Mo7O24 x 4 H2O 0.110 g 0.22 -
CuSO4 x 5 H2O 0.157 g 0.314 1.258
CoCl2 x 6 H2O 0.161 g 0.322 2.48
Distilled water 500.000 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 14.925 44.377
K2HPO4 0.846 4.856
NaH2PO4 0.746 6.22
(NH4)2SO4 0.199 1.506
MgSO4 x 7 H2O 0.07 0.283
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain