C86
86 .
RPL:PJ/0.01%RPA
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Mail sol. C86 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| RPL:PJ | fill up to 1000 | ml | - | - | |
| 0.01% RPA (Rye Grass-Prescott Agar) | fill up to 1000 | ml | - | - | |
| 1 Biphasic (see separate recipes below and overleaf) | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium C82 | 750 | ml | - | - | |
| Medium C76 | 250 | ml | - | - | |
| 1 3:1 mixture. | |||||
| 2 See separate recipes. Autoclave separately. Mix aseptically when cool. | |||||
Solution occurs in 4
other media (first defined in Medium C82).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Rye grass
(dried, milled) |
1 | g | 1 | - | |
| Medium C76 | 1000 | ml | - | - | |
| 1 Bring PJ (Mail sol. C 76) to the boil then add rye grass. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 13
other media (first defined in Medium C76).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Stock solution (CaCl2/KCl) | 1 | ml | - | - | |
|
K2HPO4
(0.51 g/100 ml stock solution) |
1 | ml | - | - | |
|
MgSO4 x 7 H2O
(0.28 g/100 ml stock solution) |
1 | ml | - | - | |
| Deionized water | 1000 | ml | - | - | |
| 1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 13
other media (first defined in Medium C76).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.43 | g | 4.3 | 29.249 |
| KCl | 0.16 | g | 1.6 | 21.462 |
Solution occurs in 1
other media (first defined in Medium C85).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Rye grass
(dried, milled) |
0.1 | g | 0.1 | - | |
| Medium C76 | 1000.0 | ml | - | - | |
|
Agar
(Oxoid L11) |
15.0 | g | 15 | - | |
| 1 Make up 1 litre of Rye GrassPrescott Liquid (RPL - see separate recipe), but use only 0.1 g of rye grass. Mix with 15 g of Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes. | |||||
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |