C82
82 .
RPL
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Mail sol. C82 #
ml
Solution occurs in 4
other media (first defined in Medium C82).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Rye grass
(dried, milled) |
1 | g | 1 | - | |
| Medium C76 | 1000 | ml | - | - | |
| 1 Bring PJ (Mail sol. C 76) to the boil then add rye grass. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 13
other media (first defined in Medium C76).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Stock solution (CaCl2/KCl) | 1 | ml | - | - | |
|
K2HPO4
(0.51 g/100 ml stock solution) |
1 | ml | - | - | |
|
MgSO4 x 7 H2O
(0.28 g/100 ml stock solution) |
1 | ml | - | - | |
| Deionized water | 1000 | ml | - | - | |
| 1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 13
other media (first defined in Medium C76).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.43 | g | 4.3 | 29.249 |
| KCl | 0.16 | g | 1.6 | 21.462 |
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Synonyms: | Rye Grass-Prescott Liquid |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |