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DSMZ

DSMZ 958 . VY/4-SWS AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Plesiocystis pacifica DSM 14875

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days aerobic 30 °C

Main sol. 958 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.0 g 20 342.231
Yeast cell paste
(bakers yeast, washed in deion. water, wet weight) 		2.5 g 2.5 -
Agar
(Bacto ) 		15.0 g 15 44.599
Cyanocobalamine
(vitamin B12) 		0.5 mg 5.0e-4 3.7e-4
Sea water salts solution 1000.0 ml - -
1 Adjust pH to 7.5 with 1M NaOH, autoclave at 121°C for 20 min. Add filter sterilized cyanocobalamine solution after autoclaving and cooling.

Sea water salts solution #

1000
Solution occurs in 1 other media (first defined in Medium 958).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferric citrate 0.01 g 0.01 0.041
MgSO4 x 7 H2O 8.00 g 8 32.458
CaCl2 x 2 H2O 1.00 g 1 6.802
KCl 0.50 g 0.5 6.707
NaHCO3 0.16 g 0.16 1.905
H3BO3 0.02 g 0.02 0.323
KBr 0.08 g 0.08 0.672
SrCl2 x 6 H2O 0.03 g 0.03 0.113
Sodium beta-glycerophosphate 0.01 g 0.01 0.046
Trace element solution SL-4 1.00 ml - -
Distilled water 1000.00 ml - -

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 6
Plesiocystis pacifica DSM 14875

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
Agar 15 44.599
MgSO4 x 7 H2O 8 32.458
Yeast cell paste 2.5 -
CaCl2 x 2 H2O 1 6.802
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Cultivation metadata from 6 strains BacDive

phyla 1 6 strains
temperature mesophilic 6 strains