Main sol. 958
Internal Solution-ID: S1975
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.0 | g | 20 | 342.231 | |
|
Yeast cell paste
(bakers yeast, washed in deion. water, wet weight) |
2.5 | g | 2.5 | - | |
|
Agar
(Bacto ) |
15.0 | g | 15 | 44.599 | |
|
Cyanocobalamine
(vitamin B12) |
0.5 | mg | 5.0e-4 | 3.7e-4 | |
| Sea water salts solution | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.5 with 1M NaOH, autoclave at 121°C for 20 min. Add filter sterilized cyanocobalamine solution after autoclaving and cooling. | |||||
Media with this solution
| 958 | VY/4-SWS AGAR |