MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 958 . VY/4-SWS AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Enhygromyxa salina DSM 15201

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 958 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.0 g 20 342.231
Yeast cell paste
(bakers yeast, washed in deion. water, wet weight) 		2.5 g 2.5 -
Agar
(Bacto ) 		15.0 g 15 44.599
Cyanocobalamine
(vitamin B12) 		0.5 mg 5.0e-4 3.7e-4
Sea water salts solution 1000.0 ml - -
1 Adjust pH to 7.5 with 1M NaOH, autoclave at 121°C for 20 min. Add filter sterilized cyanocobalamine solution after autoclaving and cooling.

Sea water salts solution #

1000
Solution occurs in 1 other media (first defined in Medium 958).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferric citrate 0.01 g 0.01 0.041
MgSO4 x 7 H2O 8.00 g 8 32.458
CaCl2 x 2 H2O 1.00 g 1 6.802
KCl 0.50 g 0.5 6.707
NaHCO3 0.16 g 0.16 1.905
H3BO3 0.02 g 0.02 0.323
KBr 0.08 g 0.08 0.672
SrCl2 x 6 H2O 0.03 g 0.03 0.113
Sodium beta-glycerophosphate 0.01 g 0.01 0.046
Trace element solution SL-4 1.00 ml - -
Distilled water 1000.00 ml - -

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 6
Enhygromyxa salina DSM 15201

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
Agar 15 44.599
MgSO4 x 7 H2O 8 32.458
Yeast cell paste 2.5 -
CaCl2 x 2 H2O 1 6.802
Show more
CSV
JSON

Cultivation metadata from 6 strains BacDive

phyla 1 6 strains
temperature mesophilic 6 strains