934
934 .
THERMOCOCCUS WAIOTAPUENSE MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thermococcus waiotapuensis DSM 12768
This strain uses the standard medium without modifications.
Growth conditions: 85 °C
Main sol. 934 #
ml
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.500 | g | 1.5 | 25.667 | |
| Na2SO4 | 4.000 | g | 4 | 28.161 | |
| KCl | 0.700 | g | 0.7 | 9.39 | |
| NaHCO3 | 0.200 | g | 0.2 | 2.381 | |
| KBr | 0.100 | g | 0.1 | 0.84 | |
| H3BO3 | 0.030 | g | 0.03 | 0.485 | |
| MgCl2 | 5.800 | g | 5.8 | 60.918 | |
| CaCl2 | 1.500 | g | 1.5 | 13.516 | |
| SrCl2 | 0.025 | g | 0.025 | 0.158 | |
| Tryptone | 5.000 | g | 5 | 70.343 | |
| Yeast extract | 1.000 | g | 1 | - | |
| Na2S x 9 H2O | 0.750 | g | 0.75 | 3.123 | |
| Resazurin | 2.000 | mg | 0.002 | 0.009 | |
| Sulphur | 10.000 | g | 10 | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 pH 7.0 | |||||
| 2 Prepare the medium anaerobically under N2. Steam the medium for 3 hours on three successive days. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Thermococcus waiotapuensis DSM 12768
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulphur | 10 | - |
| MgCl2 | 5.8 | 60.918 |
| Tryptone | 5 | 70.343 |
| Na2SO4 | 4 | 28.161 |
| NaCl | 1.5 | 25.667 |
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