# 934: THERMOCOCCUS WAIOTAPUENSE MEDIUM


## Main sol. 934

1.5	g	NaCl	
4	g	Na2SO4	
0.7	g	KCl	
0.2	g	NaHCO3	
0.1	g	KBr	
0.03	g	H3BO3	
5.8	g	MgCl2	
1.5	g	CaCl2	
0.025	g	SrCl2	
5	g	Tryptone	
1	g	Yeast extract	
0.75	g	Na2S x 9 H2O	
2	mg	Resazurin	
10	g	Sulphur	
1000	ml	Distilled water	

1. pH 7.0
2. Prepare the medium anaerobically under N2. Steam the medium for 3 hours on three successive days.