934 .
THERMOCOCCUS WAIOTAPUENSE MEDIUM
What is this page?
Selected strain: Thermococcus waiotapuensis DSM 12768
This strain uses the standard medium without modifications.
Growth conditions: 85 °C
Equipment needed:
Anaerobic workbench
Final gas composition:
100 % N2
read more
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 1.500 | g | ||
|
|
Na2SO4 | 4.000 | g | ||
|
|
KCl | 0.700 | g | ||
|
|
NaHCO3 | 0.200 | g | ||
|
|
KBr | 0.100 | g | ||
|
|
H3BO3 | 0.030 | g | ||
|
|
MgCl2 | 5.800 | g | ||
|
|
CaCl2 | 1.500 | g | ||
|
|
SrCl2 | 0.025 | g | ||
|
|
Tryptone | 5.000 | g | ||
|
|
Yeast extract | 1.000 | g | ||
|
|
Na2S x 9 H2O | 0.750 | g | ||
|
|
Resazurin | 2.000 | mg | ||
|
|
Sulphur | 10.000 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 pH 7.0 | |||||
| 2 Prepare the medium anaerobically under N2. Steam the medium for 3 hours on three successive days. | |||||