MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 92a . MODIFIED MEDIUM 92

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 92a #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 92 990 ml - -
Vitamin solution 10 ml - -
1 Prepare medium 92 and subsequently add vitamins. Adjust pH to 7.0 - 7.2

Vitamin solution #

1000
Solution occurs in 3 other media (first defined in Medium 11a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.00 mg 0.002 0.008
Folic acid 2.00 mg 0.002 0.005
Pyridoxine hydrochloride 10.00 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.00 mg 0.005 0.013
Riboflavin 5.00 mg 0.005 0.013
Nicotinic acid 5.00 mg 0.005 0.041
Calcium pantothenate 5.00 mg 0.005 0.01
p-Aminobenzoic acid 1.00 mg 1.0e-3 0.007
Vitamin B12 0.01 mg 1.0e-5 7.4e-6
Distilled water 1000.00 ml - -

Medium 92 #

1000
Solution occurs in 1 other media (first defined in Medium 92).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth 30 g 30 -
Yeast extract 3 g 3 -
Agar , if necessary 15 g 15 44.599
Distilled water 1000 ml - -
1 Adjust pH to 7.0 - 7.2.

Metadata

Last modified: 11.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase soy broth 29.7 -
Agar 14.85 44.153
Yeast extract 2.97 -
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Nicotinic acid 5.0e-5 4.1e-4
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen facultative anaerobe 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain