Name: ALKALIPHILIC THERMOCOCCUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 926 #

Equipment needed: Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	27.70	g	13.85	236.995
MgSO₄ x 7 H₂O	7.00	g	3.5	14.2
MgCl₂ x 6 H₂O	5.50	g	2.75	13.527
KCl	0.65	g	0.325	4.359
NaBr	0.10	g	0.05	0.486
NaHCO₃	0.32	g	0.16	1.905
K₂HPO₄	1.00	g	0.5	2.871
CaCl₂ x 2 H₂O	0.05	mg	2.5e-5	1.7e-4
KI	15.00	mg	0.008	0.029
H₃BO₃	0.03	g	0.015	0.243
Modified Wolin's mineral solution II	20.00	ml	-	-
Distilled water	2000.00	ml	-	-
1 Prepare the medium anaerobically under nitrogen. Do not adjust the pH.
2 Prepare separate anaerobic stock solutions of, Casamino acids(10%), Yeast extract (10%), and glycine (2M = 150 g/l). A 0.5M polysulphide solution is prepared by dissolving 12.0 g Na₂S x 9 H₂O in oxygen free water, followed by adding 1.6 g sulphur - the solution will be dark yellow.

Modified Wolin's mineral solution II #

1000

Solution occurs in 23 other media (first defined in Medium 700).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.500	g	1.5	7.848
MgSO₄ x 7 H₂O	3.000	g	3	12.172
MnSO₄ x H₂O	0.500	g	0.5	2.958
NaCl	1.000	g	1	17.112
FeSO₄ x 7 H₂O	0.100	g	0.1	0.36
CoSO₄ x 7 H₂O	0.180	g	0.18	1.161
CaCl₂ x 2 H₂O	0.100	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.180	g	0.18	0.626
CuSO₄ x 5 H₂O	0.010	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.020	g	0.02	0.042
H₃BO₃	0.010	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.010	g	0.01	0.041
NiCl₂ x 6 H₂O	0.025	g	0.025	0.193
Na₂SeO₃ x 5 H₂O	0.300	mg	3.0e-4	0.001
Distilled water	1000.000	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

All strains for this medium:	2
Strains with modifications:	2

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