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DSMZ

DSMZ 88 . SULFOLOBUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Acidianus sp. DSM 29099

* Modification: Use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 - 3.0.

Growth conditions: 3-7 days 65 °C

Main sol. 88 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.287 9.741
KH2PO4 0.28 g 0.277 2.037
MgSO4 x 7 H2O 0.25 g 0.248 1.004
CaCl2 x 2 H2O 0.07 g 0.069 0.471
FeCl3 x 6 H2O 0.02 g 0.02 0.073
Allen's trace element solution 10.00 ml - -
Yeast extract*
(OXOID) 		0.20 g - -
D-Glucose* 1.00 g - -
Sulfur powder* 10.00 g - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH.

Allen's trace element solution #

1000
Solution occurs in 14 other media (first defined in Medium 88).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 180 mg 0.18 0.91
Na2B4O7 x 10 H2O 450 mg 0.45 -
ZnSO4 x 7 H2O 22 mg 0.022 0.077
CuCl2 x 2 H2O 5 mg 0.005 0.029
Na2MoO4 x 2 H2O 3 mg 0.003 0.012
VOSO4 x 2 H2O 3 mg 0.003 0.015
CoSO4 x 7 H2O 1 mg 1.0e-3 0.006
Distilled water 1000 ml - -
1 Adjust pH of final solution to 2 with 1 N HCl.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 2.5 - 3.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 18
Strains with modifications: 17
Acidianus sp. DSM 29099

  2.5 - 3.0
  Yeast extract
  1.00 g D-Glucose
  10.00 g Sulfur powder
  0.20 g Yeast extract

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Sulfur powder 9.901 38.6
(NH4)2SO4 1.287 9.741
D-Glucose 0.99 5.496
KH2PO4 0.277 2.037
MgSO4 x 7 H2O 0.248 1.004
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Cultivation metadata from 18 strains BacDive

pH 4 - 6 2 strains
pH 8 - 10 2 strains
pH 6 - 8 1 strain
pH 10 - 12 1 strain
phyla 4 18 strains
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