Strain modifications for Medium 88
13 distinct modifications were found.
prepare without Allen’s trace element solution and Yeast extract and add per liter medium: 1ml trace element solution SL-10 from DSMZ Medium 320, 0.6g betaine hydrochloride, 10 ml vitamin solution Kao and Michayluk (Sigma-Aldrich), 1.0 g Beef extract (BD) and 1.0 g tryptone (BD),
Modified strains
Adjust pH of medium to 3.0 - 3.5.
Modified, with 0.5% yeast extract; solidified by 0.7% Gelrite; pH:9.0
Omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium.
Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium.
Supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium. Adjust the pH of the medium to 3.0.
Supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0.
Supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5.
Use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts.
Use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150°C overnight. Adjust the pH of the medium to 0.8.
Use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 - 3.0.
Use only 0.50 g/l yeast extract and supplement medium with 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust the pH of the final medium to 2.5.