Main sol. 88
Internal Solution-ID: S154
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.287 | 9.741 | |
| KH2PO4 | 0.28 | g | 0.277 | 2.037 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.248 | 1.004 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.069 | 0.471 | |
| FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.073 | |
| Allen's trace element solution | 10.00 | ml | - | - | |
|
Yeast extract
(OXOID) |
1.00 | g | 0.99 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. | |||||
Media with this solution
| 88 | SULFOLOBUS MEDIUM |