888
888 .
AQUASPIRILLUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Simplicispira metamorpha DSM 12826
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Main sol. 888 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.0 | g | 1 | 7.568 | |
| MgSO4 x 7 H2O | 1.0 | g | 1 | 4.057 | |
| CaCl2 x 6 H2O | 30.0 | mg | 0.03 | 0.137 | |
| Na2HPO4 | 10.0 | mg | 0.01 | 0.07 | |
| Sodium succinate | 1.0 | g | 1 | 6.171 | |
|
Casamino acids
(DIFCO) |
1.5 | g | 1.5 | - | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.0 | ml | - | - | |
|
Agar
(DIFCO) |
0.5 | g | 0.5 | 1.487 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH 7.5 | |||||
| 2 Add after autoclaving sterile solutions of: | |||||
|
Na2S2O3 x 5 H2O
(10% solution) |
1.0 | ml | 0.1 | 0.403 | |
| Vitamine mixture | 1.0 | ml | - | - | |
Solution occurs in 28
other media (first defined in Medium 1369).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.20 | g | 2.2 | 7.913 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.03 | g | 0.03 | 0.485 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 3.0-4.0 | |||||
Solution occurs in 2
other media (first defined in Medium 1615).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Acidic thiamine / Ca pantothenate solution | 100 | ml | - | - | |
| Basic vitamin solution | 100 | ml | - | - | |
| Neutral folic acid / riboflavin solution | 100 | ml | - | - | |
| Neutral B12 solution 0.005 g/L | 100 | ml | - | - | |
| 1 Sterilize by filtration. | |||||
| 2 Mix the four solutions in the ratio 1:1:1:1. | |||||
Solution occurs in 2
other media (first defined in Medium 1615).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Thiamine | 5 | mg | 0.05 | 0.188 |
| Calcium pantothenate | 5 | mg | 0.05 | 0.105 |
|
Distilled water
(acidified to pH 3.0 with HCl) |
100 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium 1615).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2 | mg | 0.02 | 0.082 |
|
PABA
(p-aminobenzoic acid) |
5 | mg | 0.05 | 0.365 |
| Nicotinic acid | 5 | mg | 0.05 | 0.406 |
| Pyridoxine | 10 | mg | 0.1 | 0.591 |
|
NaOH
(0.1N) |
100 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium 1615).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Folic acid | 2 | mg | 0.02 | 0.045 |
| Riboflavin | 5 | mg | 0.05 | 0.133 |
| Distilled water | 100 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium 1615).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| B12 | 0.5 | mg | 0.005 | 0.004 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Simplicispira metamorpha DSM 12826
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Casamino acids | 1.5 | - |
| (NH4)2SO4 | 1 | 7.568 |
| Sodium succinate | 1 | 6.171 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Agar | 0.5 | 1.487 |
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