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DSMZ

DSMZ 1369 . ECTOTHIORHODOSPIRA MAGNA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1369 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.5 g 0.5 3.674
NaCl 30.0 g 30 513.347
NH4Cl 0.5 g 0.5 9.348
MgCl2 x 6 H2O 0.2 g 0.2 0.984
Na-acetate 1.0 g 1 12.19
Na2S2O3 0.5 g 0.5 1.167
Na2S x 9 H2O 0.5 g 0.5 2.082
NaHCO3 5.0 g 5 59.519
Na2CO3 5.0 g 5 47.175
Yeast extract 0.1 g 0.1 -
Vitamin B12 10.0 µg 1.0e-5 7.4e-6
Trace elements solution (Pfennig, 1965) 1.0 ml - -
Distilled water 1000.0 ml - -
1 pH 9.0-9.5
2 Prepare the medium without the NaHCO3, Na2S x 9 H2O, Na2CO3 and vitamin B12, under a nitrogen atmosphere.
3 Autoclave and add the vitamin B12 from a filter-sterilised stock solution and the NaHCO3 (autoclaved in sealed, half full vessels), Na2S x 9 H2O, Na2CO3 from sterile stock solutions. The final pH of the medium should be 9.0-9.5.

Trace elements solution (Pfennig, 1965) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0 - 9.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
NaHCO3 5 59.519
Na2CO3 5 47.175
Na-acetate 1 12.19
NH4Cl 0.5 9.348
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain