MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 878 . THERMUS 162 MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Thermus igniterrae DSM 12459

This strain uses the standard medium without modifications.

Growth conditions: 70 °C

Main sol. 878 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.0 g 1 -
Tryptone 1.0 g 1 14.069
Agar 28.0 g 28 83.252
Nitrilotriacetic acid 100.0 mg 0.1 0.523
CaSO4 x 2 H2O 40.0 mg 0.04 0.232
MgCl2 x 6 H2O 200.0 mg 0.2 0.984
Fe(III) citrate
(0.01 M) 		0.5 ml 0.001 0.005
Trace element solution 0.5 ml - -
Phosphate buffer 100.0 ml - -
Distilled water 900.0 ml - -
1 Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min.
2 Autoclave the phosphate buffer separately and then add to the medium.

Phosphate buffer #

1000
Solution occurs in 3 other media (first defined in Medium 630).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 5.44 g 5.44 39.975
Na2HPO4 x 12 H2O 43.00 g 43 302.904
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 878).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2SO4 0.50 ml - -
MnSO4 x H2O 2.28 g 2.28 13.49
ZnSO4 x 7 H2O 0.50 g 0.5 1.739
H3BO3 0.50 g 0.5 8.085
CuSO4 x 5 H2O 25.00 mg 0.025 0.1
Na2MoO4 x 2 H2O 25.00 mg 0.025 0.103
CoCl2 x 6 H2O 45.00 mg 0.045 0.347
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 32
Strains with modifications: 2
Thermus igniterrae DSM 12459

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 28 83.252
Na2HPO4 x 12 H2O 4.3 30.29
Tryptone 1 14.069
Yeast extract 1 -
KH2PO4 0.544 3.998
Show more
CSV
JSON

Cultivation metadata from 32 strains BacDive

pH 6 - 8 3 strains
pH 10 - 12 2 strains
pH 4 - 6 2 strains
pH 8 - 10 1 strain
phyla 4 32 strains
Show more