780
780 .
MIDDLEBROOK MEDIUM WITH MYCOBACTIN
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 780 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mycobactine J | 2 | mg | 0.002 | - | |
| Absolute ethanol | 4 | ml | - | - | |
| Medium 645 | 1000 | ml | - | - | |
| 1 Reconstitute the contents of one vial of 2 mg Mycobactine J with 2 ml absolute ethanol (analytical grade). Shake carefully so that the product is completely dissolved. Pour the contents of the bottle into 1 litre of medium 645 kept melted in a water bath at +56°C, taking care to pour out all of the product. Rinse out the vial that contained the Mycobactine J with an additional 2 ml of absolute ethanol and pour into the culture medium. Mix the Mycobactine J alcohol solution and the culture medium very thoroughly. | |||||
Solution occurs in 1
other media (first defined in Medium 645).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Bacto Middlebrook 7H10 agar
(Difco 262710) |
19 | g | 20.995 | - | |
| Glycerol | 5 | ml | - | - | |
| Distilled water | 900 | ml | - | - | |
| 1 Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly. | |||||
| 2 If you can´t order the commercial media then prepare the media in this way: | |||||
Metadata
| Last modified: | 24.11.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Bacto Middlebrook 7H10 agar | 20.995 | - |
| Mycobactine J | 0.002 | - |
| Absolute ethanol | - | - |
| Glycerol | - | - |
| Distilled water | - | - |
