{"medium":{"id":780,"name":"MIDDLEBROOK MEDIUM WITH MYCOBACTIN","complex_medium":"yes","source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium780.pdf"},"solutions":[{"id":1585,"name":"Main sol. 780","volume":1000,"recipe":[{"recipe No.":1,"compound":"Mycobactine J","compound_id":2139,"amount":2,"unit":"mg","g_l":0.002,"optional":"no"},{"recipe No.":2,"compound":"Absolute ethanol","compound_id":788,"amount":4,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"Main sol. 645","solution_id":1369,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Reconstitute the contents of one vial of 2 mg Mycobactine J with 2 ml absolute ethanol (analytical grade). Shake carefully so that the product is completely dissolved. Pour the contents of the bottle into 1 litre of medium 645 kept melted in a water bath at +56\u00b0C, taking care to pour out all of the product. Rinse out the vial that contained the Mycobactine J with an additional 2 ml of absolute ethanol and pour into the culture medium. Mix the Mycobactine J alcohol solution and the culture medium very thoroughly."}],"main solution":"yes"},{"id":1369,"name":"Main sol. 645","volume":905,"recipe":[{"recipe No.":1,"compound":"Bacto Middlebrook 7H10 agar","compound_id":635,"attribute":"Difco 262710","amount":19,"unit":"g","g_l":20.9945,"optional":"no"},{"recipe No.":2,"compound":"Glycerol","compound_id":72,"amount":5,"unit":"ml","optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":900,"unit":"ml","optional":"no"}],"steps":[{"step":"Sterilize for 15 min by 121\u00b0C cool to 50 - 55\u00b0C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly."},{"step":"If you can\u00b4t order the commercial media then prepare the media in this way:"}],"main solution":"no"}]}