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Main sol. 645
Internal Solution-ID: S1369
This solution is used in 2 media and was first defined in medium 645.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Bacto Middlebrook 7H10 agar
(Difco 262710) |
19 | g | 20.995 | - | |
| Glycerol | 5 | ml | - | - | |
| Distilled water | 900 | ml | - | - | |
| 1 Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly. | |||||
| 2 If you can´t order the commercial media then prepare the media in this way: | |||||