63a
63a .
DESULFOTOMACULUM (14.5) MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 63a #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 980 | ml | - | - | |
| Solution B | 10 | ml | - | - | |
| Solution C | 10 | ml | - | - | |
| 1 Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas atmospherer into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Check medium pH after autoclaving and adjust to 6.8 - 7.0, if necessary. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| K2HPO4 | 0.5 | g | 0.51 | 2.929 |
| NH4Cl | 1.0 | g | 1.02 | 19.077 |
| Na2SO4 | 1.0 | g | 1.02 | 7.184 |
| CaCl2 x 2 H2O | 0.1 | g | 0.102 | 0.694 |
| MgSO4 x 7 H2O | 2.0 | g | 2.041 | 8.28 |
| Na-acetate | 2.0 | g | 2.041 | 24.878 |
| Na-pyruvate | 5.0 | g | 5.102 | 46.364 |
| Yeast extract | 1.0 | g | 1.02 | - |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 |
| Distilled water | 980.0 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.5 | g | 50 | 179.846 |
| Distilled water | 10.0 | ml | - | - |
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na-thioglycolate | 0.1 | g | 10 | 87.643 |
| Ascorbic acid | 0.1 | g | 10 | 56.78 |
| Distilled water | 10.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na-pyruvate | 5 | 45.436 |
| Na-acetate | 2 | 24.38 |
| MgSO4 x 7 H2O | 2 | 8.114 |
| NH4Cl | 1 | 18.695 |
| Na2SO4 | 1 | 7.04 |
| Show more | ||
