Main sol. 63a
Internal Solution-ID: S94
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 980 | ml | - | - | |
| Solution B | 10 | ml | - | - | |
| Solution C | 10 | ml | - | - | |
| 1 Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas atmospherer into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Check medium pH after autoclaving and adjust to 6.8 - 7.0, if necessary. | |||||
Media with this solution
| 63a | DESULFOTOMACULUM (14.5) MEDIUM |