Name: MARINE BROTH (ANAEROB)
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 514e #

Equipment needed: Autoclave Hungate tubes Gassing station

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Peptone (BD Bacto)	5.00	g	5	-
Yeast extract (BD Bacto)	1.00	g	1	-
NaCl	19.45	g	19.45	332.82
MgCl₂ (anhydrous)	5.90	g	5.9	61.968
Na₂SO₄	3.24	g	3.24	22.811
KCl	0.55	g	0.55	7.378
NaHCO₃	0.16	g	0.16	1.905
KBr	0.08	g	0.08	0.672
SrCl2	34.00	mg	0.034	0.214
H₃BO₃	22.00	mg	0.022	0.356
Na-silicate	4.00	mg	0.004	0.033
NaF (0.1% w/v)	2.40	ml	0.002	0.057
(NH₄)NO₃ (0.1% w/v)	1.60	ml	0.002	0.02
Na₂HPO₄ (0.1% w/v)	8.00	ml	0.008	0.056
Sodium resazurin (0.1% w/v)	0.50	ml	5.0e-4	-
L-Cysteine HCl x H₂O	0.30	g	0.3	1.708
Na₂S x 9 H₂O	0.30	g	0.3	1.249
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients except cysteine and sulfide, then sparge medium with 100% N₂ gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N₂ gas. Prior to use check pH of complete medium and adjust to 7.3 - 7.5, if necessary.

Last modified:	04.04.22
Source:	DSMZ
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.3 - 7.5
Equipment needed:	Autoclave, Gassing station, Hungate tubes

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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

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123	MARINE AGAR
514	BACTO MARINE BROTH (DIFCO 2216)
a	HALF STRENGTH BACTO MARINE BROTH
b	MEDIUM 514 plus additional salt
c	REACTIVATION WITH LIQUID MEDIUM 514
d	MODIFIED BACTO MARINE BROTH
e	MARINE BROTH (ANAEROB)
f	MARINE BROTH (ROTH CP73)
g	HALF STRENGTH BACTO MARINE BROTH with 50% Seawater
760	MARINE THERMOCOCCUS MEDIUM
1173	MODIFIED MEDIUM 514

514e
514e . MARINE BROTH (ANAEROB)