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DSMZ

DSMZ 457 . MINERAL MEDIUM (BRUNNER)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Sphingomonas sp. DSM 12401

* Modification: With 0.5 g/l 3-chlorosalicylic acid; Rehydrate and grow freeze-dried cells from ampoule in liquid medium 1 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates and in medium 457 with 3-chlorosalicylate.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C

Main sol. 457 #

ml
Solution occurs in 6 other media (first defined in Medium 457).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 2.44 g 2.44 17.188
KH2PO4 1.52 g 1.52 11.17
(NH4)2SO4 0.50 g 0.5 3.784
MgSO4 x 7 H2O 0.20 g 0.2 0.811
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Trace element solution SL-4 10.00 ml - -
3-Chlorosalicylic acid* 0.50 g/l - -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.9.
2 Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling.
3 Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source.

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Medium 1 #

1000
Solution occurs in 20 other media (first defined in Medium 1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , for solid medium 15 g 15 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Medium 220 #

1000
Solution occurs in 22 other media (first defined in Medium 220).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g - -
Soy peptone 5 g - -
NaCl 5 g - -
Agar 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).

Medium 535 #

1000
Solution occurs in 18 other media (first defined in Medium 535).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459) 		30 g - -
Agar 20 g - -
Distilled water 1000 ml - -
1 pH 7.3
2 Autoclave at 121°C for 15 min.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 156
Strains with modifications: 154
Sphingomonas sp. DSM 12401

  0.5 g/l 3-Chlorosalicylic acid

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 2.44 17.188
KH2PO4 1.52 11.17
(NH4)2SO4 0.5 3.784
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.05 0.34
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Cultivation metadata from 156 strains BacDive

pH 6 - 8 3 strains
pH 8 - 10 2 strains
phyla 3 156 strains
oxygen aerobe 23 strains
oxygen obligate aerobe 15 strains
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