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Main sol. 457
Internal Solution-ID: S913
This solution is used in 7 media and was first defined in medium 457.
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 | 2.44 | g | 2.44 | 17.188 | |
| KH2PO4 | 1.52 | g | 1.52 | 11.17 | |
| (NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| Trace element solution SL-4 | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 6.9. | |||||
| 2 Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling. | |||||
| 3 Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source. | |||||