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DSMZ

DSMZ 428 . HETEROTROPHIC MEDIUM H3P

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Azospirillum thiophilum DSM 21654

This strain uses the standard medium without modifications.

Growth conditions: 1-2 days aerobic 28 °C

Main sol. 428 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 50 ml - -
Solution B 855 ml - -
Solution C 20 ml - -
Solution D 30 ml - -
Solution E 50 ml - -
Standard vitamin solution 5 ml - -
1 H3P is a heterotrophic medium for growth, purity checking and isolation of a broad spectrum of aerobic bacteria (Ref. 3367).
2 Solutions A, B, C and D are autoclaved separately for 15 min at 121°C, cooled down to 50°C and then mixed aseptically with filter-sterilized solution E (at 50°C) and 5.0 ml of standard vitamin solution. The final pH of this medium should be 6.8 without adjustment.

Solution A #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.3 g 46 338.024
Na2HPO4 x 2 H2O 2.9 g 58 408.569
Distilled water 50.0 ml - -

Solution B #

855
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1.17 21.866
MgSO4 x 7 H2O 0.50 g 0.585 2.373
CaCl2 x 2 H2O 0.01 g 0.012 0.08
MnCl2 x 4 H2O 5.00 mg 0.006 0.03
NaVO3 x H2O 5.00 mg 0.006 0.042
Trace element solution SL-6 5.00 ml - -
Distilled water 850.00 ml - -
Agar , if required 20.00 g 23.392 -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferric ammonium citrate 0.05 g 2.5 -
Distilled water 20.00 ml - -

Solution D #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1 g 33.333 -
Na-acetate 1 g 33.333 406.337
Disodium succinate 1 g 33.333 205.698
DL-Malate 1 g 33.333 248.589
Distilled water 30 ml - -
1 pH 7.0

Solution E #

50
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-lactate 1 g 20 178.476
Na-pyruvate 1 g 20 181.745
D-Mannitol 1 g 20 109.788
D-Glucose 2 g 40 222.025
Distilled water 50 ml - -
1 pH 7.0
2 filter-sterilized

Standard vitamin solution #

100
Solution occurs in 3 other media (first defined in Medium 428).
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Riboflavin 10.0 mg 0.1 0.266
Thiamine-HCl x 2 H2O 50.0 mg 0.5 1.339
Nicotinic acid 50.0 mg 0.5 4.061
Pyridoxine hydrochloride 50.0 mg 0.5 2.431
Calcium pantothenate 50.0 mg 0.5 1.049
Biotin 0.1 mg 1.0e-3 0.004
Folic acid 0.2 mg 0.002 0.005
Vitamin B12 1.0 mg 0.01 0.007
Distilled water 100.0 ml - -
1 Filter sterilize (0.2 µm pore-size) and store at 4°C.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 11.10.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 109
Strains with modifications: 1
Azospirillum thiophilum DSM 21654

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 19.802 -
Na2HPO4 x 2 H2O 2.871 20.226
KH2PO4 2.277 16.734
D-Glucose 1.98 10.991
Na-pyruvate 0.99 8.997
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Cultivation metadata from 109 strains BacDive

pH 6 - 8 6 strains
pH 8 - 10 2 strains
pH 10 - 12 2 strains
phyla 4 111 strains
oxygen aerobe 14 strains
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