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Standard vitamin solution
Internal Solution-ID: S864
This solution is used in 4 media and was first defined in medium 428.
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Riboflavin | 10.0 | mg | 0.1 | 0.266 | |
| Thiamine-HCl x 2 H2O | 50.0 | mg | 0.5 | 1.339 | |
| Nicotinic acid | 50.0 | mg | 0.5 | 4.061 | |
| Pyridoxine hydrochloride | 50.0 | mg | 0.5 | 2.431 | |
| Calcium pantothenate | 50.0 | mg | 0.5 | 1.049 | |
| Biotin | 0.1 | mg | 1.0e-3 | 0.004 | |
| Folic acid | 0.2 | mg | 0.002 | 0.005 | |
| Vitamin B12 | 1.0 | mg | 0.01 | 0.007 | |
| Distilled water | 100.0 | ml | - | - | |
| 1 Filter sterilize (0.2 µm pore-size) and store at 4°C. | |||||