35
35 .
ACIDITHIOBACILLUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acidithiobacillus sulfuriphilus DSM 105150
* Modification: Adjust pH of complete medium to 3.0.
Growth conditions: 3-7 days
28 °C
Main sol. 35 #
ml
Equipment needed: Autoclave
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.10 | g | 0.1 | 1.87 | |
| KH2PO4 | 3.00 | g | 3 | 22.045 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
|
Sulfur
(powdered) |
10.00 | g | 10 | 311.818 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve all ingredients, except the sulfur and adjust the pH to 4.2, then autoclave. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. | |||||
| 2 Note: Incubate statically without shaking. | |||||
Metadata
| Last modified: | 31.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | * 3.0 |
| Equipment needed: | Autoclave, Water bath |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 10 | 311.818 |
| KH2PO4 | 3 | 22.045 |
| CaCl2 x 2 H2O | 0.14 | 0.952 |
| NH4Cl | 0.1 | 1.87 |
| MgCl2 x 6 H2O | 0.1 | 0.492 |
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