35 .
ACIDITHIOBACILLUS MEDIUM
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* Medium for Acidithiobacillus sulfuriphilus DSM 105150
Adjust pH of complete medium to 3.0.
Growth conditions: 3-7 days
28 °C
Equipment needed:
Autoclave
Water bath
Final pH:
* 3.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 0.10 | g | ||
|
|
KH2PO4 | 3.00 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.14 | g | ||
|
|
Sulfur
(powdered) |
10.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve all ingredients, except the sulfur and adjust the pH to 4.2, then autoclave. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. | |||||
| 2 Note: Incubate statically without shaking. | |||||