# 35: ACIDITHIOBACILLUS MEDIUM


## Main sol. 35

0.1	g	NH4Cl	
3	g	KH2PO4	
0.1	g	MgCl2 x 6 H2O	
0.14	g	CaCl2 x 2 H2O	
10	g	Sulfur (powdered)	
1000	ml	Distilled water	

1. Dissolve all ingredients, except the sulfur and adjust the pH to 4.2, then autoclave. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium.
2. Note: Incubate statically without shaking.