Equipment needed: Autoclave
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.10 | g | 0.1 | 1.87 | |
| KH2PO4 | 3.00 | g | 3 | 22.045 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
|
Sulfur
(powdered) |
10.00 | g | 10 | 311.818 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve all ingredients, except the sulfur and adjust the pH to 4.2, then autoclave. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. | |||||
| 2 Note: Incubate statically without shaking. | |||||
Media with this solution
| 35 | ACIDITHIOBACILLUS MEDIUM |