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DSMZ

DSMZ 357 . FLEXIBACTER CANADENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Thermoflexibacter ruber DSM 9560

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 357 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.1 g 0.1 0.406
KNO3 0.1 g 0.1 0.989
CaCl2 x 2 H2O 0.1 g 0.1 0.68
Sodium glycerophosphate 0.1 g 0.1 -
Trace element solution SL-10 1.0 ml - -
Tris 1.0 g 1 8.255
Thiamine-HCl x 2 H2O 1.0 mg 1.0e-3 0.003
Casamino acids 1.0 g 1 -
Glucose
(sterilized separately) 		1.0 g 1 5.551
Vitamin B12 1.0 µg 1.0e-6 7.4e-7
Agar 10.0 g 10 29.733
Distilled water 1000.0 ml - -
1 Adjust pH to 7.5.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 08.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3
Thermoflexibacter ruber DSM 9560

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 10 29.733
Tris 1 8.255
Glucose 1 5.551
Casamino acids 1 -
KNO3 0.1 0.989
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen facultative anaerobe 1 strain
temperature mesophilic 3 strains