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TREPONEMA SACCHAROPHILUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Bacteroides luti DSM 26991
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 323 #
ml
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.12 | g | 0.12 | 0.815 | |
| MgSO4 x 7 H2O | 0.19 | g | 0.19 | 0.769 | |
| KH2PO4 | 0.45 | g | 0.449 | 3.3 | |
| K2HPO4 | 0.45 | g | 0.449 | 2.578 | |
| NaCl | 0.90 | g | 0.898 | 15.37 | |
| (NH4)2SO4 | 0.90 | g | 0.898 | 6.797 | |
|
Yeast extract
(OXOID) |
2.00 | g | 1.996 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 1.996 | - | |
| Fatty acid mixture | 1.40 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 1.00 | g | 0.998 | 5.682 | |
| Na2CO3 | 2.50 | g | 2.495 | 23.541 | |
| D-Glucose | 2.00 | g | 1.996 | 11.079 | |
|
Agar
, for solid medium
(BD Bacto) |
12.00 | g | 11.976 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine, carbonate and glucose), adjust pH to 7.0 and sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the cysteine and carbonate, then equilibrate the medium with the CO2 gas to pH 7.0. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary. | |||||
| 2 For solid medium add 12.00 g/l agar (BD Bacto). | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| n-Butyric acid | 5.8 | ml | - | - | |
| iso-Butyric acid | 5.8 | ml | 275.5 | 3126.77 | |
| DL-2-Methylbutyric acid | 2.8 | ml | - | - | |
| n-Valeric acid | 2.8 | ml | 131.46 | 1287.18 | |
| iso-Valeric acid | 2.8 | ml | 130.34 | 1276.22 | |
| 1 Store fatty acid mixture at -20°C. | |||||
Metadata
| Last modified: | 28.08.25 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 7 |
Bacteroides luti DSM 26991
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 11.976 | - |
| Na2CO3 | 2.495 | 23.541 |
| D-Glucose | 1.996 | 11.079 |
| Trypticase peptone | 1.996 | - |
| Yeast extract | 1.996 | - |
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