Name: TREPONEMA SACCHAROPHILUM MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 323 #

Equipment needed: Autoclave Hungate tubes Filter Gassing station

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
CaCl₂ x 2 H₂O	0.12	g	0.12	0.815
MgSO₄ x 7 H₂O	0.19	g	0.19	0.769
KH₂PO₄	0.45	g	0.449	3.3
K₂HPO₄	0.45	g	0.449	2.578
NaCl	0.90	g	0.898	15.37
(NH₄)₂SO₄	0.90	g	0.898	6.797
Yeast extract (OXOID)	2.00	g	1.996	-
Trypticase peptone (BD BBL)	2.00	g	1.996	-
Fatty acid mixture	1.40	ml	-	-
Sodium resazurin (0.1% w/v)	0.50	ml	5.0e-4	0.002
L-Cysteine HCl x H₂O	1.00	g	0.998	5.682
Na₂CO₃	2.50	g	2.495	23.541
D-Glucose	2.00	g	1.996	11.079
Agar , for solid medium (BD Bacto)	12.00	g	11.976	-
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients (except cysteine, carbonate and glucose), adjust pH to 7.0 and sparge medium with 100% CO₂ gas for 30 - 45 min to make it anoxic. Add the cysteine and carbonate, then equilibrate the medium with the CO₂ gas to pH 7.0. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose from an anoxic stock solution prepared under 100% N₂ gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary.
2 For solid medium add 12.00 g/l agar (BD Bacto).

Fatty acid mixture #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
n-Butyric acid	5.8	ml	-	-
iso-Butyric acid	5.8	ml	275.5	3126.77
DL-2-Methylbutyric acid	2.8	ml	-	-
n-Valeric acid	2.8	ml	131.46	1287.18
iso-Valeric acid	2.8	ml	130.34	1276.22
1 Store fatty acid mixture at -20°C.

Last modified:	28.08.25
Source:	DSMZ
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.0
Equipment needed:	Autoclave, Filter, Gassing station, Hungate tubes

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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

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