271
271 .
ACIDITHIOBACILLUS (APH) MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acidithiobacillus sp. DSM 24413
* Modification: Omit the ferrous sulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Adjust pH of complete medium to 2.5.
Growth conditions: 3-7 days
45 °C
Main sol. 271 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 2.00 | g | 2 | 15.136 | |
| K2HPO4 | 0.50 | g | 0.5 | 2.871 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| Ca(NO3)2 x 4 H2O | 0.02 | g | 0.02 | 0.085 | |
| FeSO4 x 7 H2O* | 8.00 | g | 8 | 28.775 | |
| Elemental sulfur* | 10.00 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml 0.1 N H2SO4, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere). | |||||
| 2 Note: Incubate statically without shaking. | |||||
Metadata
| Last modified: | 13.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | * 2.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Elemental sulfur | 10 | - |
| (NH4)2SO4 | 2 | 15.136 |
| K2HPO4 | 0.5 | 2.871 |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
| KCl | 0.1 | 1.341 |
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