{"medium":{"id":271,"name":"ACIDITHIOBACILLUS (APH) MEDIUM","complex_medium":"no","min_pH":2,"max_pH":2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium271.pdf"},"solutions":[{"id":536,"name":"Main sol. 271","volume":1000,"recipe":[{"recipe No.":1,"compound":"(NH4)2SO4","compound_id":27,"amount":2,"unit":"g","g_l":2,"mmol_l":15.1355,"optional":"no"},{"recipe No.":2,"compound":"K2HPO4","compound_id":10,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.87066,"optional":"no"},{"recipe No.":3,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.02859,"optional":"no"},{"recipe No.":4,"compound":"KCl","compound_id":61,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":1.34138,"optional":"no"},{"recipe No.":5,"compound":"Ca(NO3)2 x 4 H2O","compound_id":213,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.0846923,"optional":"no"},{"recipe No.":6,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":8,"unit":"g","g_l":8,"mmol_l":28.7754,"optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml 0.1 N H2SO4, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere)."},{"step":"Note: Incubate statically without shaking."}],"equipment":{"1743":"Filter","3735":"Autoclave"},"main solution":"yes"}]}