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Main sol. 271

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Internal Solution-ID: S536

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 2.00 g 2 15.136
K2HPO4 0.50 g 0.5 2.871
MgSO4 x 7 H2O 0.50 g 0.5 2.029
KCl 0.10 g 0.1 1.341
Ca(NO3)2 x 4 H2O 0.02 g 0.02 0.085
FeSO4 x 7 H2O 8.00 g 8 28.775
Distilled water 1000.00 ml - -
1 Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml 0.1 N H2SO4, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere).
2 Note: Incubate statically without shaking.
271 ACIDITHIOBACILLUS (APH) MEDIUM